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In selecting chemical cleaning solutions for dental prostheses, compatibility between them and the type of denture base materials must be considered to avoid adverse effects on the hardness of the acrylic resin in different curing systems. This study aimed to compare the effect of different disinfectant and denture cleanser on the surface hardness of the light and heat cured acrylic resin materials. Eighty specimens are made from two different denture base materials. Forty specimens are made of light cured acrylic and forty specimens are made of heat cured acrylic resin. Each material is subdivided into four subgroups according to the type of the disinfectant (0.12% chlorhexidine digluconate, 0.5% sodium hypochlorite), the denture cleansers (Corega) and compared to the distilled water as control group. The surface hardness test is measured for each specimen to show the effect of each chemical cleaning solution on the light and heat cured denture base hardness. The results of the present study showed non significant differences in the surface hardness comparing between the two curing systems light and heat cured acrylic resin. Also there are non significant differences in the surface hardness when different disinfectant and denture cleansers have been used in comparison to the distilled water. It was concluded from this study that the hardness of acrylic materials is not affected by immersion in any type of disinfectant and denture cleanser as well as it is found that there is no different in the hardness of the light cured when compared to the heat cured acrylic denture base.