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To check the antioxidant action of Annona muricata leaf extracts and to validate its therapeutic efficacy DPPH, ABTS, Hydrogen peroxide, Hydroxyl, Nitric oxide, reducing power assay, MTT dye reduction assay, and cell cycle analysis by flow cytometry were performed. DPPH assay revealed that methanol extract showed 82.05% scavenging activity at 500µg concentration. In ABTS radical scavenging activity, ethyl acetate extract exhibited 91.03% scavenging activity at 500µg concentration. Ethanol extract actively exhibit the highest scavenging action against H2O2 radicals; the scavenging action was found to be 94.10% at 500µg concentration. Hydroxyl scavenging assay revealed that aqueous extract showed a 93.50% scavenging activity at 500µg concentration. Nitric oxide radical scavenging assay revealed that methanol extract showed 94.53% scavenging activity at 500µg concentration. Reducing power ability results showed that aqueous extracts were able to mitigate Fe 3+ ions in a dose-dependent manner, and maximum action was seen to be at 500µg concentration. MTT assay reveals that aqueous extract showed better activity, and the IC50 value was found to be at 195.42 µg concentration, the IC50 values of methanol and ethanol extracts were 318µg and 398.40 µg, respectively. Cell cycle analysis reveals that aqueous, methanol, and ethanol extract-treated group was arrested at S phase. The results suggest that Annona muricata leaves extract is an encouraging applicant for the treatment of oxidant relate d diseases and deserves additional research as an alternative to conventional drugs.