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The corporate significance of knowledge has already been proven on the basis of several research findings. Manufacturing companies play an important role in development of knowledge-based society. The added value provided by service companies is essential in knowledge creation. The success of knowledge sharing is influenced by many internal and external factors. The quality of services also depends on knowledge strategy of the company. We have focused primarily on the analysis of knowledge creation of businesses providing services in different cultures (sometimes with similar cultural features). We have analysed the corporate culture of knowledge-based organizations, as well as the corporate practice of knowledge supporting organizations in the Slovak-Hungarian border.