查看文章

The non‐steroidal anti‐inflammatory drugs (NSAIDs) are widely used for analgesia but may inhibit bone formation. We investigated whether the reported NSAID effect on bone is related to inhibition of bone marrow mesenchymal stem cell (MSC) proliferation and osteogenic and chondrogenic differentiation and evaluated both cyclooxygenase (COX)‐1 and COX‐2 specific drugs. The effects of seven COX‐1 and COX‐2 inhibitors on MSC proliferation and osteogenic and chondrogenic differentiation were tested using Vybrant, sodium 3′‐[1‐(phenylaminocarbonyl)‐ 3,4‐tetrazolium]‐bis (4‐methoxy‐6‐nitro) benzene sulfonic acid hydrate (XTT), functional and quantitative assays of MSC differentiation. The MSC expression of COX‐1 and COX‐2 and prostaglandin E2 (PGE‐2) levels were evaluated serially during lineage differentiation by quantitative PCR and ELISA. None of the NSAIDs at broad range of …