作者
Antje Ebert, Amit U Joshi, Sandra Andorf, Yuanyuan Dai, Shrivatsan Sampathkumar, Haodong Chen, Yingxin Li, Priyanka Garg, Karl Toischer, Gerd Hasenfuss, Daria Mochly-Rosen, Joseph C Wu
发表日期
2019/6/21
期刊
Circulation research
卷号
125
期号
1
页码范围
90-103
出版商
Lippincott Williams & Wilkins
简介
Rationale:
The immature presentation of human induced pluripotent stem cell–derived cardiomyocytes (iPSC-CMs) is currently a challenge for their application in disease modeling, drug screening, and regenerative medicine. Long-term culture is known to achieve partial maturation of iPSC-CMs. However, little is known about the molecular signaling circuitries that govern functional changes, metabolic output, and cellular homeostasis during long-term culture of iPSC-CMs.
Objective:
We aimed to identify and characterize critical signaling events that control functional and metabolic transitions of cardiac cells during developmental progression, as recapitulated by long-term culture of iPSC-CMs.
Methods and Results:
We combined transcriptomic sequencing with pathway network mapping in iPSC-CMs that were cultured until a late time point, day 200, in comparison to a medium time point, day 90, and an early …
学术搜索中的文章
A Ebert, AU Joshi, S Andorf, Y Dai, S Sampathkumar… - Circulation research, 2019