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Cell division and expansion have a pivotal importance for enhancement of efficient metabolite production through cell cycle regulation in transcriptional levels. The Gap1/Synthesis (G1/S) transition of the cell cycle could predict the products for synthesis as well as the distribution of the secondary metabolite between the cell and the medium with cessation of cell cycle in this transition. In this study, 1000 bp upstream sequences of G1/S transition genes as promoter regions from Arabidopsis thaliana were analyzed to introduce new generation of pharmaceutical metabolite stimuli. Identification and analysis of potential cis-regulatory motifs with using Botany Array Resource database (BAR) detected ten cis-regulatory elements that had significant differences than other cis-regulatory elements. Then, we evaluated the function of ten Cis-regulatory elements with PlantCARE database. TGACG-motif as Methyl jasmonate …