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The present investigation deals with evaluation of hepatoprotective and antioxidant activity of total methanol extract and different fractions of Odontonema cuspidatum on the CCl4 induced hepatotoxicity.

Albino Wister rats weighing (140±20 g) of either sex were divided into seven groups (n=8). Hepatotoxicity was induced by CCl4 dissolved in olive oil (1:1, 2 ml/kg bw, S.c). Silymarin (100mg/kg) orally was used as standard drug. Test groups received total methanol extract and different fractions (aqueous, n-butanol and n-hexane fractions) of Odontonema cuspidatum aerial partin a dose of 400 mg/kg/day orally along with CCl4. Treatment was given to all the tested groups daily for 5 days. The hepatoprotective effect of Odontonema cuspidatum was evaluated by assessment of biochemical parameters [(AST), (ALT), (ALP) and total bilirubin] and the antioxidant activity in the liver tissue was estimated by measuring the activities of antioxidant enzymes: reduced glutathione (GSH) as well as the level of lipid peroxidation by Malondialdehyde (MDA). Biochemical observations were also supported with histopathological examination of liver section.

Odontonema cuspidatum methanol extract (OCME) (400mg/kg) exhibited a highly significant reduction (p