作者
MS Refaey, MAH Mustafa, AM Mohamed, AA Ali
发表日期
2015
期刊
Journal of Pharmacognosy and Phytochemistry
卷号
4
期号
2
页码范围
89-96
出版商
AkiNik Publications
简介
Objective
The present investigation deals with evaluation of hepatoprotective and antioxidant activity of total methanol extract and different fractions of Odontonema cuspidatum on the CCl4 induced hepatotoxicity.
Material and methods
Albino Wister rats weighing (140±20 g) of either sex were divided into seven groups (n=8). Hepatotoxicity was induced by CCl4 dissolved in olive oil (1:1, 2 ml/kg bw, S.c). Silymarin (100mg/kg) orally was used as standard drug. Test groups received total methanol extract and different fractions (aqueous, n-butanol and n-hexane fractions) of Odontonema cuspidatum aerial partin a dose of 400 mg/kg/day orally along with CCl4. Treatment was given to all the tested groups daily for 5 days. The hepatoprotective effect of Odontonema cuspidatum was evaluated by assessment of biochemical parameters [(AST), (ALT), (ALP) and total bilirubin] and the antioxidant activity in the liver tissue was estimated by measuring the activities of antioxidant enzymes: reduced glutathione (GSH) as well as the level of lipid peroxidation by Malondialdehyde (MDA). Biochemical observations were also supported with histopathological examination of liver section.
Results
Odontonema cuspidatum methanol extract (OCME) (400mg/kg) exhibited a highly significant reduction (p
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MS Refaey, MAH Mustafa, AM Mohamed, AA Ali - Journal of Pharmacognosy and Phytochemistry, 2015