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Limonene, a mono‐terpene flavonoid, has anti‐inflammatory effects in asthma by activating A_2A adenosine receptors. We studied the effects of inhaled d‐limonene on isolated aortic and tracheal rings from asthmatic mice using tissue/organ bath system. We used mice in which the A_2A receptor was genetically knocked out (A_2AKO) and C57BLJ/6 (wild‐type; WT) for the study. The mice were divided into control (CON) and allergen‐induced sensitized (SEN), control mice treated with limonene through aerosol route (CON+LIM) and allergen‐induced sensitized mice treated with limonene (SEN+LIM). Mice were sensitized i.p. on days 1, 6 with 20μg ovalbumin (OVA) followed by 5% OVA aerosol challenges on days 11–13. Control groups were given an i.p injection of vehicle (1,6 days) and the aerosol challenge of normal saline (11–13 days). SEN +LIM and CON +LIM groups were given 0.01% limonene in the form of …