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The primary aim of the present study was to evaluate the potential of P. hysterophorus weed biomass valorization through its utilization in the production of cellulase enzyme.

The work involved bio-prospecting of fungal microbes and selecting the most potent fungal isolate for its cellulolytic potential. Thereafter, endoglucanase production by the fungal isolate was enhanced by utilizing P. hysterophorus biomass as a substrate. The optimization study involved the production of the enzyme under both stationary as well as shaking conditions. A scale-up for the enzyme production was also carried out under batch mode of fermentation in Erlenmeyer flasks as well as in a 2 l-bioreactor.

Penicillium citrinum NAF5, isolated from garden soil, was found as a potent cellulase producer. Under optimized conditions, P. citrinum NAF5 produced 4.224 ± 0.035 U/ml and 3.13 ± 0.026 U/ml …