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Traditional fluorescent sensors for simultaneous detection of metal ions require the use of specially designed fluorescent probes. In this manuscript, we have employed a new strategy for the design of a sensor for simultaneous detection of metal ions that is based on a fluorescent probe encapsulated hydrogel matrix. An investigation designed to assess this strategy led to the development of a novel dual fluorescent encapsulated hydrogel microsphere sensor for sensitive and simultaneous detection of Al³⁺ and Hg²⁺. The hyperbranched poly (poly (ethylene glycol) diacrylate) (HB-PEGDA) and thiolated sodium alginate (SA-SH) composite hydrogel microspheres used to construct the sensor, which contain two fluorescent probes encapsulated in a highly dense manner, were prepared by using a self-assembly method in conjunction with a microfluidic device. The functionalized hydrogel microspheres were shown to …