作者
Suliman A Alsagaby, Rajendran Vijayakumar, Mariappan Premanathan, Suresh Mickymaray, Wael Alturaiki, Raid S Al-Baradie, Saleh AlGhamdi, Mohammad A Aziz, Fahad A Alhumaydhi, Faisal A Alzahrani, Ameen S Alwashmi, Waleed Al Abdulmonem, Naif Khalaf Alharbi, Chris Pepper
发表日期
2020/10/13
期刊
International Journal of Nanomedicine
页码范围
7901-7921
出版商
Taylor & Francis
简介
Introduction
Zinc oxide nanoparticles (ZnO NPs) have recently attracted attention as potential anti-cancer agents. To the best of our knowledge, the toxicity of ZnO NPs against human chronic myeloid leukemia cells (K562 cell line) has not been studied using transcriptomics approach.
Objective
The goals of this study were to evaluate the capability of ZnO NPs to induce apoptosis in human chronic myeloid leukemia cells (K562 cells) and to investigate the putative mechanisms of action.
Methods
We used viability assay and flowcytometry coupled with Annexin V-FITC and propidium iodide to investigate the toxicity of ZnO NPs on K562 cells and normal peripheral blood mononuclear cells. Next we utilized a DNA microarray-based transcriptomics approach to characterize the ZnO NPs-induced changes in the transcriptome of K562 cells.
Results
ZnO NPs exerted a selective toxicity (mainly by apoptosis) on the leukemic …
学术搜索中的文章
SA Alsagaby, R Vijayakumar, M Premanathan… - International Journal of Nanomedicine, 2020