作者
Prasad Kottayil Padmanabhan, Gabriel Reis Ferreira, Ouafa Zghidi-Abouzid, Camila Oliveira, Carole Dumas, Filipe Colaço Mariz, Barbara Papadopoulou
发表日期
2021/9/20
期刊
Nucleic Acids Research
卷号
49
期号
16
页码范围
9459-9478
出版商
Oxford University Press
简介
DDX3 is a multifaceted RNA helicase of the DEAD-box family that plays central roles in all aspects of RNA metabolism including translation initiation. Here, we provide evidence that the Leishmania DDX3 ortholog functions in post-initiation steps of translation. We show that genetic depletion of DDX3 slows down ribosome movement resulting in elongation-stalled ribosomes, impaired translation elongation and decreased de novo protein synthesis. We also demonstrate that the essential ribosome recycling factor Rli1/ABCE1 and termination factors eRF3 and GTPBP1 are less recruited to ribosomes upon DDX3 loss, suggesting that arrested ribosomes may be inefficiently dissociated and recycled. Furthermore, we show that prolonged ribosome stalling triggers co-translational ubiquitination of nascent polypeptide chains and a higher recruitment of E3 ubiquitin ligases and proteasome components to ribosomes …
学术搜索中的文章
PK Padmanabhan, GR Ferreira, O Zghidi-Abouzid… - Nucleic Acids Research, 2021