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The clustered, regularly interspaced short palindromic repeats (CRISPR) and their cooperation with CRISPR-associated (Cas) genes has become a powerful genome engineering tool. The CRISPR/Cas system was discovered as a part of the adaptive immune system in bacteria and archaea to defend from plasmids and phages. CRISPR/Cas9 protein has become the most favored editing tool in recent years. CRISPR/Cas restriction system involves the restriction of endonuclease enzyme guided by a hybrid strand of RNA consisting of CRISPR RNA (crRNA) and trans-activating RNA (tracrRNA) that results in a gene knock-out or knock-in followed by non-homologous end joining (NHEJ) and homology-directed repair (HDR). Owing to its efficiency, specificity, and reproducibility, apart from its application in biotechnology, CRISPR/Cas has been explored for its therapeutic potential in several human diseases. However …