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To determine the effects of oxidative stress on the quality of oocytes and embryos, 8-hydroxy-2′-deoxyguanosine (8-OHdG) in granulosa cells was quantitatively studied during an in vitro fertilization and embryo transfer (IVF-ET) program.

Immunocytochemical staining of 8-OHdG in granulosa cells was quantitatively estimated using a charge-coupled device camera and analyzed using the National Institute of Health Image (NIH Image) freeware on a computer.

Obstetrics and gynecology department in a university hospital. Patient(s): Ninety-six infertile couples undergoing IVF-ET treatment and intracytoplasmic sperm injection (IVF, n = 72; intracytoplasmic sperm injection, n = 24). Intervention(s): Oocytes, granulosa cells, and follicular fluids were collected 35–36 hours after the administration of hCG. Main Outcome Measure(s): 8-OHdG indices were obtained for mural [8-OHdG index (m)] and …