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Dehydrins (DHNs) have been reported to act as chaperones to combat drought stress. While in this study, we explored a novel nuclear complex of Oryza sativa FKBP and YSK2-type dehydrin (OsDhn-Rab16D), associated with ABA signaling to impart drought stress tolerance. The transcript levels of OsDhn-Rab16D in rice seedlings were induced in response to drought, abscisic acid (ABA) and H₂O₂ exposure. Ectopic expression of OsDhn-Rab16D transgenic lines showed enhanced tolerance to both osmotic stresses caused by PEG and drought. Using the yeast two-hybrid (Y2H) assay, O. sativa FKBP (a prolyl cis-trans isomerase) was identified as an interacting partner of OsDhn-Rab16D. Fluorescence signals between OsDhn-Rab16D and OsFKBP were observed in the nucleus. The pull-down assay confirmed the physical interaction between OsDhn-Rab16D and OsFKBP. qRT-PCR of drought and ABA …