作者
Ping Lu, Isaiah L Hankel, Judit Knisz, Andreas Marquardt, Ming-Yi Chiang, Johannes Grosse, Rainer Constien, Thomas Meyer, Andreas Schroeder, Lutz Zeitlmann, Umaima Al-Alem, Ann D Friedman, Eric I Elliott, David K Meyerholz, Thomas J Waldschmidt, Paul B Rothman, John D Colgan
发表日期
2010/7/7
期刊
The Journal of experimental medicine
卷号
207
期号
7
页码范围
1359
出版商
The Rockefeller University Press
简介
A recessive mutation named Justy was found that abolishes B lymphopoiesis but does not impair other major aspects of hematopoiesis. Transplantation experiments showed that homozygosity for Justy prevented hematopoietic progenitors from generating B cells but did not affect the ability of bone marrow stroma to support B lymphopoiesis. In bone marrow from mutant mice, common lymphoid progenitors and pre-pro–B cells appeared normal, but cells at subsequent stages of B lymphopoiesis were dramatically reduced in number. Under culture conditions that promoted B lymphopoiesis, mutant pre-pro–B cells remained alive and began expressing the B cell marker CD19 but failed to proliferate. In contrast, these cells were able to generate myeloid or T/NK precursors. Genetic and molecular analysis demonstrated that Justy is a point mutation within the Gon4-like (Gon4l) gene, which encodes a protein with …
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P Lu, IL Hankel, J Knisz, A Marquardt, MY Chiang… - The Journal of experimental medicine, 2010