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Medicinal plants have played an essential role in human health for generations. In District Multan, traditional knowledge about these plants provides a rich resource, yet it remains largely undocumented and may be in danger of fading with younger generations. This study aimed to document the medicinal plant knowledge in District Multan examining the diversity of plants used, methods of preparation, parts utilized and the relationship between plants and ailments with insights from diverse methodologies like interviews and observations. Among the 339 participants, 75% were male, with those aged 51–60 years having a profound knowledge of herbal practices suggesting a decline in the passing down of this knowledge. A significant wild plant diversity was evident, with 14 distinct plant families found, Solanaceae being predominant. Of the studied categorized plants, herbs emerged as the most prevalent (59 …