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Five flavonoid glucuronides were obtained from the fruit of Helicteres isora, three of which were previously unknown compounds: isoscutellarein 4′-methyl ether 8-O-β-d-glucuronide 6″-n-butyl ester, isoscutellarein 4′-methyl ether 8-O-β-d-glucuronide 2″, 4″-disulfate and isoscutellarein 8-O-β-d-glucuronide 2″,4″-disulfate. The structures were determined on the basis of spectroscopy and hydrolysis experiments.