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Particulate matter (PM) is a criteria pollutant emitted from animal feeding operation (AFO) facilities, especially from poultry operation buildings. Fundamental data regarding AFO PM either do not exist, or are not representative of different animal production systems or housing types. This field study investigated particle size distributions (PSDs) and concentrations of total suspended particulate (TSP) in a tunnel ventilated high-rise layer house under different operational conditions. Six low-volume (1m³h⁻¹) TSP samplers were used to collect PM samples on two floors of the high-rise layer houses across four seasons through day/night sampling protocols. The day/night sampling design was to examine animal activity impact. The PM samples were analyzed by a multi-wave length laser diffraction particle size analyzer (LS13 320) for PSDs characterized by mass median diameters (MMDs) and geometric standard …