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This article reports two years of quality-assured measurements of concentrations and baseline emissions of ammonia (NH₃) at two tunnel-ventilated high-rise houses (houses 3 and 4) located at an egg production facility in North Carolina. The study was conducted as part of the National Air Emissions Monitoring Study (NAEMS). The inside NH₃ concentrations, as represented by the exhaust air, were characterized by significant diurnal and seasonal variations. The lowest exhaust concentrations (17.0 ±14.0 ppm in house 3; 15.8 ±13.0 ppm in house 4) were observed in early afternoon (i.e., 2:00 p.m. to 3:00 p.m.) at the maximum diurnal ventilation rate. The highest concentrations (29.5 ±22.7 ppm in house 3; 28.8 ±22.2 ppm in house 4) occurred in early morning (i.e., 2:00 a.m. to 3:00 a.m.) when the ventilation rate was the lowest. Similarly, the concentrations were lowest during summer and maximum ventilation …