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Given the energy crisis, fossil fuel reserves crisis, climate mitigation, and energy efficiency increase, scientists have embarked on producing alternative fuels such as the biodiesel. This study was conducted to investigate the feasibility of biodiesel production from safflower oil using the ultrasonic system, and to evaluate the produced fuel using a diesel power generator. In this study, the effects of alcohol-to-oil molar ratio, ultrasound power (W), catalyst concentration (w/w %), and the reaction time (min) on methyl ester yield were investigated. By increasing the molar ratio to a point between the ratios 4:1 and 6:1, the conversion rate first increases 11.42%, and then it remains unchanged from the point 6 to 8. As the ultrasonic power increases, the rate of conversion increases incrementally. The optimization was obtained at 7.02 molar ratio, 160 W ultrasound power, 0.95 (w/w%) catalyst concentration, and 8.47 min …