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India ranks number one in terms of incidence and prevalence rates for Tuberculosis across the globe and it is a major healthcare problem faced by government. Lot of efforts had been taken to control the spread of the Tuberculosis, still the rates are uncontrolled and every year incidence and prevalence rates are increasing. The major cause of spread of this disease is undiagnosed and untreated patients, due to lack of India specific healthcare facilities at primary healthcare centers. Conventional methods are restricted to tertiary healthcare setups due to high cost and specific operating conditions. Present study addresses evaluation of India specific indigenous and portable TB detection system and conducted from June 2019 to December 2019, in Central Research Laboratory (CRL), MGM Medical College and Hospital, Kamothe, Navi Mumbai, India. The sensitivity and specificity of the developed system was compared with conventional TB diagnostic tools such as Real Time PCR and Smear Microscopy technique. In the present study the sensitivity and specificity of DNA Detection system was found to be 100% and 97% as compared to 100% sensitivity as well as specificity of Real Time PCR assay and 63.16% sensitivity and 100% specificity of most commonly used smear microscopy technique. The developed device can be suitable for screening of infectious diseases at rural setup with conventional PCR in time bound and cost effective manner.