查看文章

Nutritionally, shrimps are rich in proteins to man and also contribute to income generation worldwide. Unfortunately, cases of mortality of shrimps have been reported in countries where shrimps aquaculture is practiced. Vibrio species have presented serious health problems to shrimps and humans. This study, sought to investigate the occurrence and antibiotics susceptibility pattern of Vibrio species from shrimps: Parapaeopsis atlantica, Penaeus notialis and Penaeus kerathurus. Shrimp samples (121) were obtained from the Kribi coastal water, Cameroon. 0.1 ml of 103 dilutions of a prepared homogenate was used to inoculate thiosulphate citrate bile salt sucrose agar plates. Presumptive identification of isolates was done through morphological and biochemical tests, genetically confirmed by polymerase chain reactions-restriction fragment length polymorphism (PCR-RFLP). Agar disc diffusion method was used to obtain inhibition zones of antibiotics. Of the 121 shrimp samples, 73 (60.03%) were contaminated with Vibrio spp. Five different Vibrio species were identified. Vibrio alginolyticus (46.2%) record the highest prevalence and Vibrio spp, the least (32.2%). Vibrio parahaemolyticus and V. alginolyticus were present in all shrimp samples. The PCR-RFLP identified all isolates analysed as Vibrio species. The phylogenetic tree showed V. alginolyticus strains split into 2 clearly different Clades which are Clade 1 and Clade 2 suggesting two population structures. Contrary to the banding pattern of V. parahaemolyticus 2 and V. alginolyticus 5, suggesting the same genotype, the two species branched in separate sub-trees. Vibrio …