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The non-peptide angiotensin II (AII) receptor subtype selective antagonist, DuP 753, was used to characterize AII receptor binding sites in the rat brain. DuP 753 competed for specific ¹²⁵I-[Sar¹, Ile⁸]AII (¹²⁵I-SIAII) binding in many brain nuclei (IC₅₀ = 20−30 nM), but was a weak competitor at remaining sites (IC₅₀ > 10⁻⁴ M). DuP 753 sensitive binding sites (designated AII_α subtype) correspond with areas where binding is inhibited by sulfhydryl reducing agents, whereas DuP 753 insensitive sites (AII_β) correspond with areas where binding is not inhibited by sulfhydryl reducing agents.