作者
Frank-Peter Wachs, Sebastien Couillard-Despres, Maren Engelhardt, Daniel Wilhelm, Sonja Ploetz, Maurice Vroemen, Johanna Kaesbauer, Goekhan Uyanik, Jochen Klucken, Claudia Karl, Johanna Tebbing, Clive Svendsen, Norbert Weidner, Hans-Georg Kuhn, Juergen Winkler, Ludwig Aigner
发表日期
2003/7
期刊
Laboratory investigation
卷号
83
期号
7
页码范围
949-962
出版商
Nature Publishing Group
简介
Neural stem cells (NSCs) from the adult central nervous system are currently being investigated for their potential use in autologous cell replacement strategies. High expansion rates of NSCs in culture are crucial for the generation of a sufficient amount of cells needed for transplantation. Here, we describe efficient growth of adult NSCs in Neurobasal medium containing B27 supplement under clonal and low-density conditions in the absence of serum or conditioned medium. Expansion of up to 15-fold within 1 week was achieved on low-density NSC cultures derived from the lateral ventricle wall, the hippocampal formation, and the spinal cord of adult rats. A 27% single-cell cloning efficiency in Neurobasal/B27 combination further demonstrates its growth-promoting ability. Multipotency and nontumorgenicity of NSCs were retained despite the high rate of culture expansion. In addition, increased cell survival was …
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FP Wachs, S Couillard-Despres, M Engelhardt… - Laboratory investigation, 2003