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Several washing agents (EDTA, CDTA, NTA, 8 HQS, Ti‐EDTA‐citrate, Ca²⁺, and H⁺) were tested for their ability to extract adsorbed metal from biological surfaces to distinguish between internalized and total cellular metal (Pb, Ni, Cd, Zn, and Cu) for a green alga, Chlorella kesslerii. Although a single extraction protocol cannot be provided that would be acceptable for all biological species and metals, several guidelines are proposed that should facilitate the interpretation of metal bioaccumulation data. It was shown that for the studied metals, a thermodynamic explanation was generally sufficient to explain the metal extraction results. At pH 6.0, a solution of 5 × 10⁻³ M ethylenediaminetetraacetic acid (EDTA) was an acceptable washing agent in most cases, whereas Ca or proton exchange solutions did not appear to quantitatively remove adsorbed metals. Conditions of ligand excess could be attained with …