作者
Kristian Ødegaard Andresen, Morten Hansen, Maria Matschuk, Søren Terpager Jepsen, Henrik Schiøtt Sørensen, Pawel Utko, Dávid Selmeczi, Thomas S Hansen, Niels B Larsen, Noemi Rozlosnik, Rafael Taboryski
发表日期
2010/4/7
期刊
Journal of Micromechanics and Microengineering
卷号
20
期号
5
页码范围
055010
出版商
IOP Publishing
简介
We present the design-concept for an all polymer injection molded single use microfluidic device. The fabricated devices comprise integrated conducting polymer electrodes and Luer fitting ports to allow for liquid and electrical access. A case study of low voltage electroporation of biological cells in suspension is presented. The working principle of the electroporation device is based on a focusing of the electric field by means of a constriction in the flow channel for the cells. We demonstrate the use of AC voltage for electroporation by applying a 1 kHz,±50 V square pulse train to the electrodes and show delivery of polynucleotide fluorescent dye in 46% of human acute monocytic leukemia cells passing the constriction.
引用总数
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学术搜索中的文章
KØ Andresen, M Hansen, M Matschuk, ST Jepsen… - Journal of Micromechanics and Microengineering, 2010