作者
Ayalew Tefferi, Jacob J Strand, Terra L Lasho, Michelle A Elliott, Chin‐Yang Li, Ruben A Mesa, Gordon W Dewald
发表日期
2006/4/15
期刊
Cancer: Interdisciplinary International Journal of the American Cancer Society
卷号
106
期号
8
页码范围
1739-1743
出版商
Wiley Subscription Services, Inc., A Wiley Company
简介
BACKGROUND
Patients who have myelofibrosis with myeloid metaplasia (MMM) display recurrent, albeit nonspecific cytogenetic abnormalities that are diverse prognostically. For the current study, the authors explored the relation between specific cytogenetic clones and JAK2V617F mutational status in patients with MMM and the effects on treatment response to erythropoietin (Epo).
METHODS
Concomitantly collected blood granulocytes and bone marrow were processed for JAK2V617F mutation analysis and cytogenetic studies, respectively. Genomic DNA was amplified by polymerase chain reaction, and fluorescent dye chemistry sequencing was performed by using the same primers that were used for amplification.
RESULTS
Among 105 study patients, cytogenetic abnormalities were detected in 47 patients (45%), and the JAK2V617F mutation was detected in 52 patients (50%). Comparison of mutational …
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A Tefferi, JJ Strand, TL Lasho, MA Elliott, CY Li… - Cancer: Interdisciplinary International Journal of the …, 2006