作者
Luke Zappia, Belinda Phipson, Alicia Oshlack
发表日期
2017/9/12
期刊
Genome biology
卷号
18
期号
1
页码范围
174
出版商
BioMed Central
简介
As single-cell RNA sequencing (scRNA-seq) technologies have rapidly developed, so have analysis methods. Many methods have been tested, developed, and validated using simulated datasets. Unfortunately, current simulations are often poorly documented, their similarity to real data is not demonstrated, or reproducible code is not available. Here, we present the Splatter Bioconductor package for simple, reproducible, and well-documented simulation of scRNA-seq data. Splatter provides an interface to multiple simulation methods including Splat, our own simulation, based on a gamma-Poisson distribution. Splat can simulate single populations of cells, populations with multiple cell types, or differentiation paths.
学术搜索中的文章
L Zappia, B Phipson, A Oshlack - Genome biology, 2017