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The physico-chemical parameters of Ado-Ekiti Reservoir in Southwest region of Nigeria were investigated from May 2002 to July 2004. Monthly mean values of the parameters were; Temperature 27.70C±1.60 (range 23.500C to 30.040C); Transparency 0.99 m±0.26 (range 0.51 to 1.54 m); pH 8.39 (range 5.63 to 9.82 mg/l); Dissolved Oxygen (DO) 5.7±1.47 mg/l (Range 4.08 to 8.98 mg/l, Biochemical Oxygen Demand (BOD) 1.90±0.61 mg/l (range 0.93 to 3.20 mg/l); Alkalinity 45±19.40 mg CaCO3/l (range 22.0 to 100.88 CaCO3/l); Free Carbon dioxide 24.61±8.50 ppm (range 16.50 to 47.50 ppm). Results showed monthly variations of the physico-chemical parameters in the reservoir. Correlation coefficients (r) for all pairs of determined physico-chemical parameters showed that transparency had a significant correlation with Dissolved Oxygen (0.52) and pH (0.63)(p≤ 0.05 and 0.01 levels). Alkalinity had a positive correlation with pH (0.43) and low negative correlation (-0.30;-0.21) with DO and Free Carbon dioxide. Abundance of