作者
JS Rajoriya, JK Prasad, SK Ghosh, P Perumal, Anuj Kumar, Shobhana Kaushal, SS Ramteke
发表日期
2014/9/1
期刊
Asian pacific journal of reproduction
卷号
3
期号
3
页码范围
192-199
出版商
No longer published by Elsevier
简介
Objective
To assess the effect of different seasons on expression of HSP70 and HSP90 genes of spermatozoa in Indian breed, Tharparkar bull.
Methods
Total numbers of 60 ejaculates from 3 bulls were collected through artificial vagina method twice a week during summer and winter season (30 ejaculates from each season). The semen samples were pooled and diluted with the standard TEYC extender and these semen samples were allowed to study the expression of HSP 70 and HSP 90 genes of spermatozoa with commercially available kit.
Results
No significant difference was observed in spermatozoal mRNA expression of HSP 70 and HSP 90 during winter and summer season in this bull semen. But the mRNA expression of both HSP 70 and HSP 90 during summer season was found non-significantly higher in comparison to winter season.
Conclusion
It was concluded from the present study that there was no …
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