作者
Mohammadreza Shariatgorji, Patrik Källback, Lena Gustavsson, Nicoletta Schintu, Per Svenningsson, Richard JA Goodwin, Per E Andren
发表日期
2012/5/15
期刊
Analytical chemistry
卷号
84
期号
10
页码范围
4603-4607
出版商
American Chemical Society
简介
The limit of detection of low-molecular weight compounds in tissue sections, analyzed by matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), was significantly improved by employing sample washing using a pH-controlled buffer solution. The pH of the washing solutions were set at values whereby the target analytes would have low solubility. Washing the tissue sections in the buffered solution resulted in removal of endogenous soluble ionization-suppressing compounds and salts, while the target compound remained in situ with minor or no delocalization during the buffered washing procedure. Two pharmaceutical compounds (cimetidine and imipramine) and one new protease inhibitor compound were successfully used to evaluate the feasibility of the pH-controlled tissue washing protocol for MALDI-MSI. Enhancement in signal-to-noise ratio was achieved by a factor of up to 10.
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M Shariatgorji, P Källback, L Gustavsson, N Schintu… - Analytical chemistry, 2012