作者
AM Irani, Timothy R Bradford, Christopher L Kepley, Norman M Schechter, Lawrence B Schwartz
发表日期
1989/10
期刊
Journal of Histochemistry & Cytochemistry
卷号
37
期号
10
页码范围
1509-1515
出版商
SAGE Publications
简介
We developed an improved immunohistochemical technique for distinguishing human mast cells of the MCT (tryptase-positive, chymase-negative) and MCTC (tryptase-positive, chymase-positive) types utilizing a biotinylated murine anti-chymase monoclonal antibody (MAb), termed B7, and an alkaline phosphatase-conjugated murine anti-tryptase MAb, termed G3. The B7 MAb also was used to show the selective presence of chymase in mast cells. The distribution of MCT and MCTC cells in Carnoy's fluid-fixed tissue sections of human lung, skin, small intestine, and tonsils was analyzed by the new technique and the results compared to those obtained with the older method using a rabbit polyclonal antichymase antibody and a mouse anti-tryptase MAb in indirect immunoperoxidase and indirect immunoalkaline phosphatase protocols, respectively. In tissues known to contain predominantly mature mast cells, there …
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AM Irani, TR Bradford, CL Kepley, NM Schechter… - Journal of Histochemistry & Cytochemistry, 1989