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Long-living memory stem T cells (T_SCM) with the ability to self-renew and the plasticity to differentiate into potent effectors could be valuable weapons in adoptive T-cell therapy against cancer. Nonetheless, procedures to specifically target this T-cell population remain elusive. Here, we show that it is possible to differentiate in vitro, expand, and gene modify in clinically compliant conditions CD8⁺ T_SCM lymphocytes starting from naive precursors. Requirements for the generation of this T-cell subset, described as CD62L⁺CCR7⁺CD45RA⁺CD45R0⁺IL-7Rα⁺CD95⁺, are CD3/CD28 engagement and culture with IL-7 and IL-15. Accordingly, T_SCM accumulates early after hematopoietic stem cell transplantation. The gene expression signature and functional phenotype define this population as a distinct memory T-lymphocyte subset, intermediate between naive and central memory cells. When transplanted in …