作者
Zhixiong Wang, Swetha Kambhampati, Yulan Cheng, Ke Ma, Cem Simsek, Alan H Tieu, John M Abraham, Xi Liu, Vishnu Prasath, Mark Duncan, Alejandro Stark, Alexander Trick, Hua-Ling Tsai, Hao Wang, Yulong He, Mouen A Khashab, Saowanee Ngamruengphong, Eun J Shin, Tza-Huei Wang, Stephen J Meltzer
发表日期
2019/4/1
期刊
Clinical Cancer Research
卷号
25
期号
7
页码范围
2127-2135
出版商
American Association for Cancer Research
简介
Purpose
Barrett's esophagus is the only known precursor of esophageal adenocarcinoma (EAC). Although endoscopy and biopsy are standard methods for Barrett's esophagus diagnosis, their high cost and risk limit their use as a screening modality. Here, we sought to develop a Barrett's esophagus detection method based on methylation status in cytology samples captured by EsophaCap using a streamlined sensitive technique, methylation on beads (MOB).
Experimental Design
We conducted a prospective cohort study on 80 patients (52 in the training set; 28 in the test set). We used MOB to extract and bisulfite-convert DNA, followed by quantitative methylation-specific PCR to assess methylation levels of 8 previously selected candidate markers. Lasso regression was applied to establish a prediction model in the training set, which was then tested on the independent test …
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Z Wang, S Kambhampati, Y Cheng, K Ma, C Simsek… - Clinical Cancer Research, 2019