作者
Jiasheng Wang, Ke Ding, Yujie Chen, Lifeng Zhang, Zukai Liu, Angli Xue, Wenjia Gu, Xiaoyue Yang, Xihan Li, Jin Huang, Congcong Xing, Yunlong Cao, Ming Chen
发表日期
2014/12/19
来源
ACS Synthetic Biology
卷号
3
期号
12
页码范围
963-965
出版商
American Chemical Society
简介
A rapid on-site detection of exogenous proteins without the need for equipped laboratories or skilled personnel would benefit many areas. We built a rapid protein detection platform based on aptamer-induced inner-membrane scaffolds dimerization by virtue of bacterial ghost system. When the detection platform was coincubated with two kinds of aptamers targeting two different sites of thrombin, green fluorescence or β-lactamase activity were yielded with two different designs. The latter was detected by commercially available testing strips.
学术搜索中的文章
J Wang, K Ding, Y Chen, L Zhang, Z Liu, A Xue, W Gu… - ACS Synthetic Biology, 2014