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This study's successful preparation of copper oxide nanoparticles (CuO NPs) involved combining a blood liquid extractor with Cu₂NO₃ salt via a hydrothermal process. CuO NPs are essential to the antibacterial process because they eradicate germs and provide a disease-free atmosphere. XRD analysis, FE-SEM images, TEM, EDX, Zeta potential, UV–visible spectra, and FT-IR spectra were employed to investigate CuO NPs. According to the XRD data, CuO NPs have an FCC structure and a crystallite size range from 9.3 to 18 nm. Diffraction peaks for CuO NPs may be assigned high crystal quality. Furthermore, the FE-SEM data showed that the CuO NPs had a tiny grain size of between 15 and 33 nm. TEM scans revealed that CuO NPs were spherical in shape and ranged in size from 11.6 to 20 nm. EDX spectra with their image showed CuO purity with Cu and O occurrences. The symmetrical stretching …