作者
Roni Ben-Ami, Agnes Klochendler, Matan Seidel, Tal Sido, Ori Gurel-Gurevich, Moran Yassour, Eran Meshorer, Gil Benedek, Irit Fogel, Esther Oiknine-Djian, Asaf Gertler, Zeev Rotstein, Bruno Lavi, Yuval Dor, Dana G Wolf, Maayan Salton, Yotam Drier, The Hebrew University-Hadassah COVID, Diagnosis Team
发表日期
2020/9/1
期刊
Clinical Microbiology and Infection
卷号
26
期号
9
页码范围
1248-1253
出版商
Elsevier
简介
Introduction
Testing for active SARS-CoV-2 infection is a fundamental tool in the public health measures taken to control the COVID-19 pandemic. Because of the overwhelming use of SARS-CoV-2 reverse transcription (RT)-PCR tests worldwide, the availability of test kits has become a major bottleneck and the need to increase testing throughput is rising. We aim to overcome these challenges by pooling samples together, and performing RNA extraction and RT-PCR in pools.
Methods
We tested the efficiency and sensitivity of pooling strategies for RNA extraction and RT-PCR detection of SARS-CoV-2. We tested 184 samples both individually and in pools to estimate the effects of pooling. We further implemented Dorfman pooling with a pool size of eight samples in large-scale clinical tests.
Results
We demonstrated pooling strategies that increase testing throughput while maintaining high sensitivity. A comparison of …
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R Ben-Ami, A Klochendler, M Seidel, T Sido… - Clinical Microbiology and Infection, 2020