作者
Michael Derevyanchuk, Raisa Litvinovskaya, Vladimir Khripach, Volodymyr Kravets
发表日期
2016/4
期刊
Plant growth regulation
卷号
78
页码范围
297-305
出版商
Springer Netherlands
简介
Using radioactive labeling technique it was analyzed proteins profiles of maize plants after treatment (12 h) with salt and brassinosteroids (BRs). Among separated proteins 66 and 67 proteins were observed specifically upregulated by salt treatment or BRs under salinity, respectively. Brassinazole—inhibitor of BRs biosynthesis—lowered BR-induced accumulation of proteins that confirm their BR-mediated synthesis. Data on 200 heat shock proteins (HSPs) from Arabidopsis thaliana and 42 HSPs from Zea mays have been used for polypeptide identification with bioinformatic approach. Protein bands separated on SDS-PAGE were compared with gene expression profile of HSPs from Genevestigator database in response to salinity and hormone treatment. In result, eight correlative matches were found between levels of polypeptide accumulation and activation of HSPs genes’ expression. It was suggested …
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