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A reliable and highly sensitive hydrogen peroxide (H₂O₂) field effect transistor (FET) sensor is reported, which was constructed by using molybdenum disulfide (MoS₂)/reduced graphene oxide (RGO). In this work, we prepared MoS₂ nanosheets by a simple liquid ultrasonication exfoliation method. After the RGO-based FET device was fabricated, MoS₂ was assembled onto the RGO surface for constructing MoS₂/RGO FET sensor. The as-prepared FET sensor showed an ultrahigh sensitivity and fast response toward H₂O₂ in a real-time monitoring manner with a limit of detection down to 1 pM. In addition, the constructed sensor also exhibited a high specificity toward H₂O₂ in complex biological matrix. More importantly, this novel biosensor was capable of monitoring of H₂O₂ released from HeLa cells in real-time. So far, this is the first report of MoS₂/RGO based FET sensor for electrical detection of signal molecules …