作者
Ivan Martinez, Karen E Hayes, Jamie A Barr, Abby D Harold, Mingyi Xie, Syed IA Bukhari, Shobha Vasudevan, Joan A Steitz, Daniel DiMaio
发表日期
2017/6/20
期刊
Proceedings of the National Academy of Sciences
卷号
114
期号
25
页码范围
E4961-E4970
出版商
National Academy of Sciences
简介
The reversible state of proliferative arrest known as “cellular quiescence” plays an important role in tissue homeostasis and stem cell biology. By analyzing the expression of miRNAs and miRNA-processing factors during quiescence in primary human fibroblasts, we identified a group of miRNAs that are induced during quiescence despite markedly reduced expression of Exportin-5, a protein required for canonical miRNA biogenesis. The biogenesis of these quiescence-induced miRNAs is independent of Exportin-5 and depends instead on Exportin-1. Moreover, these quiescence-induced primary miRNAs (pri-miRNAs) are modified with a 2,2,7-trimethylguanosine (TMG)-cap, which is known to bind Exportin-1, and knockdown of Exportin-1 or trimethylguanosine synthase 1, responsible for (TMG)-capping, inhibits their biogenesis. Surprisingly, in quiescent cells Exportin-1–dependent pri-miR-34a is present in the …
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I Martinez, KE Hayes, JA Barr, AD Harold, M Xie… - Proceedings of the National Academy of Sciences, 2017