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This investigation was carried out during 2013 and 2014 seasons on ten-years-old Superior seedless grapevines grown in a sandy loam soil at El-Khatatba, Menoufiya governorate. The objective of this investigation was to study the effect of summer pruning and some bio-stimulants on bud fertility, vegetative growth, yield and fruit quality. The vines were cultivated at 2 X 3 meters apart and irrigated by the drip irrigation system, according to cane-pruning under the Spanish Baron trills system. The treatments were applied as follows: inoculation with arbuscular mycorrhiza (AM), inoculation with yeast (Y), summer pruning (Sp), (AM) + yeast (Y), (AM) + summer pruning (Sp), yeast (Y) + summer pruning (Sp), (AM) + yeast (Y) + summer pruning (Sp) and control (Uninoculated vines). The results showed that all treatment significantly increased bud fertility percentage and Fruitful coefficient in the second season than the control. Moreover, the combination of Arbuscular mycorrhiza + Yeast + Summer pruning recorded the highest significant values of yield per vine, cluster weight as well as the physical and chemical characteristics of berries. Dynamics of wood ripening, total chlorophyll and percentages of total nitrogen, phosphorus and potassium of the leaves and total carbohydrates of the canes were also improved. Concerning, microbiological activity in the rhizosphere, it was noticed that percentage of infection of AM-mycorrhizae, spore numbers of AM-mycorrhizae, the populations of total microorganism and yeast populations increased in the rhizosphere as a result of inoculation of AM-mycorrhizae and yeast.