作者
Bote G Bruinsma, Gautham V Sridharan, Pepijn D Weeder, James H Avruch, Nima Saeidi, Sinan Özer, Sharon Geerts, Robert J Porte, Michal Heger, Thomas M van Gulik, Paulo N Martins, James F Markmann, Heidi Yeh, Korkut Uygun
发表日期
2016/3/3
期刊
Scientific reports
卷号
6
期号
1
页码范围
22415
出版商
Nature Publishing Group UK
简介
As donor organ shortages persist, functional machine perfusion is under investigation to improve preservation of the donor liver. The transplantation of donation after circulatory death (DCD) livers is limited by poor outcomes, but its application may be expanded by ex vivo repair and assessment of the organ before transplantation. Here we employed subnormothermic (21 °C) machine perfusion of discarded human livers combined with metabolomics to gain insight into metabolic recovery during machine perfusion. Improvements in energetic cofactors and redox shifts were observed, as well as reversal of ischemia-induced alterations in selected pathways, including lactate metabolism and increased TCA cycle intermediates. We next evaluated whether DCD livers with steatotic and severe ischemic injury could be discriminated from ‘transplantable’ DCD livers. Metabolomic profiling was able to cluster livers with …
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BG Bruinsma, GV Sridharan, PD Weeder, JH Avruch… - Scientific reports, 2016