作者
Xabier Osteikoetxea, Márton Benke, Marta Rodriguez, Krisztina Pálóczi, Barbara W Sódar, Zsuzsanna Szvicsek, Katalin Szabó-Taylor, Krisztina V Vukman, Ágnes Kittel, Zoltán Wiener, Károly Vékey, László Harsányi, Ákos Szűcs, Lilla Turiák, Edit I Buzás
发表日期
2018/4/30
期刊
Biochemical and biophysical research communications
卷号
499
期号
1
页码范围
37-43
出版商
Academic Press
简介
Aims
The prognosis of patients with pancreatic cancer has remained virtually unchanged with a high mortality rate compared to other types of cancers. An earlier detection would provide a time window of opportunity for treatment and prevention of deaths.
In the present study we investigated extracellular vesicle (EV)-associated potential biomarkers for pancreatic cancer by directly assessing EV size-based subpopulations in pancreatic juice samples of patients with chronic pancreatitis or pancreatic cancer. In addition, we also studied blood plasma and pancreatic cancer cell line-derived EVs.
Methods
Comparative proteomic analysis was performed of 102 EV preparations from human pancreatic juices, blood, and pancreatic cancer cell lines Capan-1 and MIA PaCa-2. EV preparations were also characterized by electron microscopy, tunable resistive pulse sensing, and flow cytometry.
Results
Here we describe the …
学术搜索中的文章
X Osteikoetxea, M Benke, M Rodriguez, K Pálóczi… - Biochemical and biophysical research communications, 2018