作者
Dawei Chen, Zhiguo Zhang, Beili Chen, Dongmei Ji, Yan Hao, Ping Zhou, Zhaolian Wei, Yunxia Cao
发表日期
2017/7/1
期刊
Biology of reproduction
卷号
97
期号
1
页码范围
91-103
出版商
Oxford University Press
简介
Diminished ovarian reserve (DOR) is defined as decreased number or quality of follicles and oocytes in a woman at childbearing age. It is estimated that up to 10% of women in the general population may suffer from DOR. This study aims to comprehensively characterize microRNA (miRNA) and Piwi-interacting RNA (piRNA) expression profiles in cumulus cells of DOR patients. Cumulus cells were collected from 20 women of similar chronological age who received assisted reproductive technology treatment: 10 with DOR and 10 with normal ovarian reserve (NOR). The small RNAs were extracted from each sample and reverse transcribed. Deep sequencing and bioinformatic analysis were performed to identify the small noncoding RNA profiles. The rRNAs were the most abundant small RNA class in cumulus cells derived from human MII oocytes, following were miRNAs and tRNAs. Twenty-six piRNAs, 79 …
引用总数
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