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Objective:

To investigate the effect of crocin carotenoid on BNDF and CREB gene expression in the brain ventral tegmental area (VTA) and the serum level of BDNF in morphine-treated rats compared to control.

Methods:

In this study, 40 male Wistar rats (200-250 g) were used in 5 experimental groups: 1) non morphine treat rats (control); 2) non morphine-treated rats with 25 mg/kg crocin carotenoid (ip, for 21 d); 3) morphine treated rats (10 mg/kg twice a day, sc, 21 d); 4 and 5) morphine-treated rats with 12.5 and 25 mg/kg crocin carotenoid, respectively. By the end of research, BDNF and CREB expression was determined by real-time-PCR method. ELISA analysis was also applied for assessing the serum BDNF level.

Results:

The data indicated that morphine treatment could cause a significant decrease in BDNF and CREB gene expression (P< 0.01 and P< 0.001, respectively) in brain VTA as well as serum level of …