作者
Sebina Shrestha, Brian E Applegate, Jesung Park, Xudong Xiao, Paritosh Pande, Javier A Jo
发表日期
2010/8/1
期刊
Optics Letters
卷号
35
期号
15
页码范围
2558-2560
出版商
Optica Publishing Group
简介
Fluorescence lifetime imaging microscopy (FLIM) offers a noninvasive approach for characterizing the biochemical composition of biological tissue. In recent years, there has been an increasing interest in the application of multispectral FLIM for medical diagnosis. Central to the clinical translation of FLIM technology is the development of robust, fast, and cost-effective FLIM instrumentation suitable for in vivo tissue imaging. Unfortunately, the predominant multispectral FLIM approaches suffer from limitations that impede the development of high-speed instruments for in vivo applications. We present a cost-effective scanning multispectral FLIM implementation capable of achieving pixel rates on the order of tens of kilohertz, which will facilitate the evaluation of FLIM for in vivoapplications.
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