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We investigated whether oscillating gradient diffusion MRI (dMRI) can provide information on brain microstructural changes after formaldehyde fixation and after hypoxic‐ischemic (HI) injury beyond that provided by conventional dMRI.

Pulsed gradient spin echo (PGSE) and oscillating gradient spin echo (OGSE) dMRI of the adult mouse brain was performed in vivo (50–200 Hz, b = 600 mm²/s), and a similar protocol was applied to neonatal mouse brains at 24 h after unilateral hypoxia‐ischemia. Animals were perfusion fixed with 4% paraformaldehyde for ex vivo dMRI and histology.

Apparent diffusion coefficients (ADCs) measured in the live adult mouse brain presented tissue‐dependent frequency‐dependence. In vivo OGSE‐ADC maps at high oscillating frequencies (>100 Hz) showed clear contrast between the molecular layer and granule cell layer in the adult mouse cerebellum …