作者
Sven Asmussen, Hiroshi Ito, Daniel L Traber, Jae W Lee, Robert A Cox, Hal K Hawkins, Daniel F McAuley, David H McKenna, Lillian D Traber, Hanjing Zhuo, Jennifer Wilson, David N Herndon, Donald S Prough, Kathleen D Liu, Michael A Matthay, Perenlei Enkhbaatar
发表日期
2014/9/1
期刊
Thorax
卷号
69
期号
9
页码范围
819-825
出版商
BMJ Publishing Group Ltd
简介
Background
Human bone marrow-derived mesenchymal stem (stromal) cells (hMSCs) improve survival in mouse models of acute respiratory distress syndrome (ARDS) and reduce pulmonary oedema in a perfused human lung preparation injured with Escherichia coli bacteria. We hypothesised that clinical grade hMSCs would reduce the severity of acute lung injury (ALI) and would be safe in a sheep model of ARDS.
Methods
Adult sheep (30–40 kg) were surgically prepared. After 5 days of recovery, ALI was induced with cotton smoke insufflation, followed by instillation of live Pseudomonas aeruginosa (2.5×1011 CFU) into both lungs under isoflurane anaesthesia. Following the injury, sheep were ventilated, resuscitated with lactated Ringer's solution and studied for 24 h. The sheep were randomly allocated to receive one of the following treatments intravenously over 1 h in one of the following groups: (1 …
学术搜索中的文章
S Asmussen, H Ito, DL Traber, JW Lee, RA Cox… - Thorax, 2014