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Benthic foraminiferal assemblages in 24 sediment surface samples collected at water depths ranging from 9 to 103 m on the continental shelf off Guadiana River were analysed, leading to the identification of a total of 270 species. Cluster analyses performed on species with a relative abundance higher than 5% led to the identification of four assemblages. The shallowest assemblage, down to 12 m water depth, is represented by Ammonia beccarii, Asterigerinata mamila, Eggerelloides scaber, Mississipina concentrica, Planorbulina mediterranensis, Elphidium crispum and Quinqueloculina laevigata. A second assemblage between 12 and 40 m water depth is characterised by Bulimina elongata, Cribrononion gerthi and Nonion fabum. The assemblage between 40 and 95 m water depth is characterised by Bolivina dilatata, Epistominella vitrea, Bulimina aculeata, Bolivina ordinaria, Spiroloxostoma croarae and …